Part:BBa_K3380150:Design
iSpinach fluorescent RNA aptamer
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We ordered the synthesized sequence. Originally ordered sequence: forward (5’TACT agacgcgactacggtgagggtcgggtccagtagcttcggctactgttgagtagagtgtgggctccgtagtcgcgtctc 3’ ) and (5’CATTgagacgcgactacggagcccacactctactcaacagtagccgaagctactggacccgaccctcaccgtagtcgcgtct 3’) with TACT and CATT respectively being a linker, to facilitate further manipulations (swapping of the part or attaching to different promoters).
The forward and reverse sequences were phosphorylated, annealed and ligated to other parts using T4 DNA ligase.
The iSpinach RNA aptamer was flanked by the F30 tRNA scaffold BBa_K3380101 and BBa_K3380102 for increased fluorescence and stability.
Source
iSpinach is an artificial RNA aptamer that was obtained using the SELEXapproach. It is based on the Spinach fluorescent RNA aptamer.
References
Autour, A., Westhof, E. and Ryckelynck, M., 2016. iSpinach: a fluorogenic RNA aptamer optimized for in vitro applications. Nucleic acids research, 44(6), pp.2491-2500.